Isolation and Identification of Human Rhinovirus from aborted Women in Iraq

Abstract

Objective: The relationship between pregnant women with Bad Obstetric History (BOH) like abortion and dead fetuses and the infection with Human Rhinovirus (HRV) for the first time.
Methods: Three hundred throat swabs were collected from pregnant
women with (BOH) from Mosul and Baghdad , extraced viral RNA from
Throat swabs specimens using QIAGEN QIAamp®Viral RNA Min
Kit.Germany .while Real-time PCR process for HRV was using (Fast-track
FTD Respiratory pathogens 33. Luxembourg). HeLa cell line used for
isolation the HRV and after the obtained the CPE and detection the HSV
using TEM.
Results: Our results showed that 10% of the collected specimen were
positive for the detection of (HRV) by Real Time PCR .The HRV were
isolated in continuous human cell lines HeLa Cells Which detected by the
appearance of characteristic cytopathic effect (CPE), which initially appears
as foci of rounded up cells. The diagnosis of HRV was confirmed by using
Transmission Electron Microscopy (TEM), which showed positive
appearance of HRV as small round in shape its size about of 30.5 nm at
200 kx.
Conclusion: There are a correlation between HRV infection with pregnant
women with BOH and which diagnosed it by Real time PCR and tissue
culture and determined it by TEM.